Oligo & DNA Resuspension Calculator

A DNA/RNA oligo resuspension calculator computes the volume of solvent (such as 1X TE buffer or nuclease-free water) required to dissolve lyophilized synthetic oligonucleotide primers to a desired molar concentration.

To use the Oligo & DNA Resuspension Calculator, enter your Oligo Amount (nmol), Desired Target Concentration (µM). The calculator instantly computes Volume of Buffer to Add, Stock Solution Concentration and more. Results update in real time as you change any input — no submit button needed.

How to Use This Calculator

Enter your Oligo Amount (nmol) (e.g. 25 in nmol) and Desired Target Concentration (µM) (e.g. 100 in µM (µmol/L)). Results update instantly as you type — no submit button or page reload required. Adjust any value to compare scenarios side by side.

The Formula

Rule of Thumb: Buffer Volume (µL) = Oligo Amount (nmol) × 10 (for 100 µM stock)

General Formula: Buffer Volume (µL) = [Oligo Amount (nmol) ÷ Target Concentration (µM)] × 1000

Worked Example

25 nmol lyophilized oligonucleotide dry pellet, desired stock concentration of 100 µM.

• Rule of Thumb: 25 nmol × 10 = 250 µL.

• Volume of Buffer to Add: 250.0 µL.

• Yields a 100 µM (0.10 mM) master stock solution.

Understanding Your Result

Evaluate your result against the specific financial decision driving this calculation. Small input differences can create large output swings — run two or three scenarios (conservative, base, optimistic) to understand the range of outcomes. For decisions involving more than a month's income, validate your result against a second source or consult a certified financial planner (CFP) or CPA depending on the tax implications.